DESCRIPTION MW= 34.2 kDa. Full length nsp5 (main protease, 3CLp, 3CL-PRO, 3C-like proteinase) from SARS-CoV-2, expressed in E. coli. The protein consists in residues 1-306 (NCBI reference sequence YP_009725301.1). Four additional residues, GSHM, are present at the N-term of the protein.
SEQUENCE Numeration of the nsp5 from NCBI YP_009725301.1
10 20 30 40 50 GSHM SGFRKMAFPS GKVEGCMVQV TCGTTTLNGL WLDDVVYCPR HVICTSEDML 60 70 80 90 100 110 NPNYEDLLIR KSNHNFLVQA GNVQLRVIGH SMQNCVLKLK VDTANPKTPK YKFVRIQPGQ 120 130 140 150 160 170 TFSVLACYNG SPSGVYQCAM RPNFTIKGSF LNGSCGSVGF NIDYDCVSFC YMHHMELPTG 180 190 200 210 220 230 VHAGTDLEGN FYGPFVDRQT AQAAGTDTTI TVNVLAWLYA AVINGDRWFL NRFTTTLNDF 240 250 260 270 280 290 NLVAMKYNYE PLTQDHVDIL GPLSAQTGIA VLDMCASLKE LLQNGMNGRT ILGSALLEDE 300 FTPFDVVRQC SGVTFQ
ISOTOPIC ENRICHMENT none
SUPPLIED AS Protein solution. The concentration is calculated from the absorbance at 280nm (ε280 = 33640 M-1cm-1).
STORAGE -20°C. After initial defrost, aliquot the product into individual tubes and refreeze at -20°C. Avoid repeated freeze/thaw cycles.
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